However, we did not see evidence of PI3K-Akt activation in BHDS-derived tumors using an expression signature that was a robust predictor of PI3K-Akt pathway activation in other renal tumors [53], nor did we see consistent enrichment of the three mTOR activation signatures from the MsigDB in the BHDS patient samples. This evidence concerns the gene AKT1 and Renal neoplasm.