Finally, we identified 9 protein spots exhibiting over 8-fold increase or decrease in abundance in replicate gels: tubulin beta-2 (spot 509), phosphoglucomutase 3 (PGM3) (spot 4620), unnamed protein product (spot 5728), smoothelin-A (spot 6011), KIAA0073 (spot 7414), Melanoma-derived leucine zipper, extra-nuclear factor (spot 8010), unnamed protein product (spot 8210), hypothetical protein LOC57691 (spot 8405), activin A type I receptor precursor (spot 8714) (Figure 3B and Table 1). The gene discussed is PPWD1; the disease is melanoma.