The density of VGF immunoreactivity was significantly lower in the lumbar anterior horns of sporadic ALS patients than those in control patients with other diseases (0.40±0.09, 0.46±0.14, and 0.57±0.12 of the control in sections C7, T8, and L4, respectively, n = 6) (Fig. 5E). This evidence concerns the gene VGF and amyotrophic lateral sclerosis.