The present study investigated the effects of mildronate in a 6-OHDA rat model of PD by assessing cellular biomarkers that are involved in signaling cascades and that are crucial for neural and glial integration: the neuronal marker TH (tyrosine hydroxylase); ubiquitin (a regulatory peptide involved in the ubiquitin-proteasome degradation system); Notch-3 (a marker of progenitor cells); IBA-1 (ionised calcium-binding adaptor molecule 1, a marker of microglia); glial fibrillary acidic protein, GFAP (a marker of astrocytes); inducible nitric oxide synthase, iNOS (a marker of inflammation). This evidence concerns the gene NOTCH3 and Parkinson disease.