Furthermore, point mutations of the downstream kinase isoform MEK1 (P124L, P124S and Q56P), have resulted in changes of the allosteric drug-binding pocket within helices A and C leading to sub-optimal drug binding of the selective RAF inhibitor PLX4720 in mutant melanoma cells in cell culture and in vivo (Emery et al, 2009). The gene discussed is RAF1; the disease is melanoma.