In the present study, we addressed this issue by using low (0.1 pfu/cell) and high (10 pfu/cell) MOIs for the infection of cultured porcine kidney epithelial cells with wild-type PRV, and subsequently analysed and compared the expressions of 37 PRV genes and two antisense transcripts (AST and LAT) using the SYBR Green-based real-time RT-PCR technique. Here, LAT is linked to infection.