To investigate mRNA expression of ECT2 identified as a cancer-related gene by our microarray analysis [6], we performed quantitative reverse transcriptase PCR (qRT-PCR) analysis using six OSCC-derived cell lines (HSC-2, HSC-3, HSC-4, H1, Ca9-22, and Sa3) and human normal oral keratinocytes (HNOKs). This evidence concerns the gene ECT2 and cancer.