While all individuals with hearing loss were homozygous for a missense mutation (F1888S) in CDH23, those subjects with the hearing loss affecting all frequencies were also heterozygous for a missense mutation (V586M) in plasma-membrane calcium pump PMCA2, which is encoded by ATP2B2. Individuals with hearing loss in the higher frequencies but with normal thresholds in the lower range had the normal 586V allele. This evidence concerns the gene ATP2B2 and hearing loss disorder.