Apoptosis was quantified by FACS analyses of cells labelled with FITC-Annexin V. Treatment of MCF-7 cells with α-TEA at 20 or 40 μM and HCC-1954 cells at 10 or 20 μM for 24 h induced both cell types to undergo apoptosis in a dose-dependent manner, with HCC-1954 cells exhibiting greater sensitivity than MCF-7 cells (Figure 1A). Here, ANXA5 is linked to hepatocellular carcinoma.