In 1997, Disis et al. [35] showed by capture enzyme-linked immunosorbent assay (ELISA) and verified by western blot analysis that serum anti-HER2 AAbs, at titers of > or = 1 : 100, were present in 12/107 (11.2%) early-stage breast carcinoma patients and in none (0%) of 200 healthy controls (P < .01). The gene discussed is ERBB2; the disease is breast carcinoma.