A recent editorial on COLD-PCR technology suggested that "The utility of COLD-PCR will be greatly enhanced if this approach can be demonstrated to completely eliminate microdissection steps or at least to substantially reduce the tumor percentage required....[16]." We show that the COLD-PCR melting curve assay is sensitive enough to readily detect a KRAS mutation without tumor dissection in a sample with only ~3% tumor cells. Here, KRAS is linked to neoplasm.