Since NF-κB is a potential target of GSK3β-dependent cell survival pathway, we detected apoptotic cells as an Annexin-V+/7-AAD+ population within DMSO or SB216763-treated malignant cells cultured ex vivo from each of the 11 patients with ALL by using Annexin-V staining and flow cytometry. The gene discussed is NFKB1; the disease is acute lymphoblastic leukemia.