PFKFB2 and acute lymphoblastic leukemia: To further address whether PFKFB2 regulation was related to GC-sensitivity and/or preB-ALL and T-ALL origin, we treated 9 leukemia cell lines with 10-7M dexamethasone for 2, 6, 12 and 24 hours in biological triplicates and performed real time RT-PCR using primers covering the exon-3/exon-4 boundary present in both splice variants (Figure 1A).