We observed that mice vaccinated intratumorally with E7 peptide in combination with PADRE peptide and poly(I:C) generated a significantly higher percentage of tumor-infiltrating CD8+ T cells (Figure 5A) as well as E7-specific CD8+ T cells (Figure 5B and 5C) in the TILs compared to mice vaccinated with the same regimen subcutaneously (* p < 0.05). This evidence concerns the gene CD8A and neoplasm.