As recent reports showed that the reduced contractility of glioma in which ACTN4 has been significantly downregulated with siACTN4 RNA was due to the decreased expression of myosin II [8], we determined the expression of myosin II in both WT ACTN4 and ACTN4 KD murine lung fibroblasts. The gene discussed is ACTN4; the disease is central nervous system cancer.