In contrast, isogenic SD transfectants expressing either scGal-deficient LPG (‘null-scGal’ SD-SSU:SCG5) or LPG with longer side chain polymers (‘oligo-scGal’ SD-SSU:SCG1, ‘poly-scGal’ SD-SSU:SCG4) survived poorly in PpapJ flies, mirroring infection outcomes with naturally-occurring L. major strains SD or LV39c5 (‘null-scGal’ or ‘poly-scGal’ LPG PAMPs, respectively). This evidence concerns the gene CHGB and infection.