ASPG and neoplasm: Now, we report that the recombinant enzyme, a type II L-asparaginase [32], [33], shows a strong preference for L-asparagine over L-glutamine, with a broad pH optimum activity in the range 7.0–10.0 and a drop of 80% activity below pH 4.0 [31] The H. pylori enzyme displayed a cytotoxic activity towards HDF cells and several tumour cell lines consistently higher than the E. coli orthologue, affecting survival in a concentration and cell-type dependent manner [31].