Next, in order to address whether the inhibition of HIF-1α by TPZ was cell line specific, we extended these studies to a diverse set of tumor cell lines with tissues of various origins, including the hepatic-cancer cell lines HepG2 and SMMC-7721, the colon-cancer cell line HCT116, the breast-cancer cell line OVCAR8 and the embryonic-kidney cell line HEK-293. This evidence concerns the gene HIF1A and breast cancer.