This might explain the earlier induction of IFN-γ and TNF-α by F3sap vaccine, its strongest efficacy (88–90.55% of reduction of parasite load) and the lower potential of the NH36 vaccine for generation of DTH, IFN-γ/IL-10 ratio of CD4+ and CD8+ T cells as well as the reduction of hepatomegaly and parasite load detected in this (37.06% and 65.90%) and in previous investigations (67.80–79.00%), respectively [14], [25]. This evidence concerns the gene CD4 and Hepatomegaly.