Figure 2B summarises the results from three independent experiments, confirming that only TSPAN8 and CAPG were significantly overexpressed in T1C3 compared with IC8 cells. Indeed, T1C3 cells exhibited an approximately 11- and 4-fold cell surface expression of TSPAN8 and CAPG, respectively, than did IC8 cells. The differential expression of these two proteins was further confirmed by western blot analysis (Figure 2C). It should be noted, however, that TSPAN8 was so weakly expressed in IC8 melanoma cells that no band could be visualised (Figure 2C). This evidence concerns the gene TSPAN8 and melanoma.