DNMT3B and malignant colon neoplasm: Knowing that CpG hypomethylation is involved in BORIS activation [2], [8], [16], we compared BORIS expression in the HCT116 colon cancer cell line to HCT116 cells treated with 5aza-dC, and to HCT116 bearing a double knockout (DKO) of DNMT3b and DNMT1[21].