Further analysis of the tumor immunohistochemistry by multivariable logistic regression corroborated the results of the Spearman's correlation analysis showing the strongest association between COX-2 expression and Akt pathway activation in ER-negative and HER2-positive breast tumors independent of age at diagnosis, disease stage, race/ethnicity, and neoadjuvant chemotherapy (Table 4). The gene discussed is ESR1; the disease is breast neoplasm.