WRAP53 and cancer: To further examine the presence of WRAP53 in Cajal bodies, a panel of cancer cell lines and primary cells including U2OS, H1299, HCT116, HEK293, MCF-7, HeLa-PV, and HDF were stained using a polyclonal antibody against WRAP53 and a monoclonal antibody against the Cajal body marker coilin.