We found that in the brains of 2-month-old mice, the steady-state levels of TDP-43 and the ~35 kDa C-terminal fragment (herein referred to as TDP-35), which can be detected by overexposing the blots, were similar between 3×Tg-AD and age- and gender-matched non transgenic (NonTg) mice (Figure 1A-B). This evidence concerns the gene TARDBP and Alzheimer disease.