We investigated the prophylactic effect of the IL-13Rα2 DNA vaccine followed by boost vaccination with ECDα2 protein mixed with adjuvants on naturally expressing IL-13Rα2 MCA304 sarcoma and 4T1 breast carcinoma tumors in C57BL/6 and BALB/c mice, respectively. The gene discussed is IL13RA2; the disease is breast carcinoma.