IL13RA2 and neoplasm: As shown in Figure 1C, overall sacrifice time (OST) of animals (tumor-bearing mice were sacrificed when tumor size reached 2 cm in diameter according to NIH animal guidelines) was 23 days in VR mock vaccinated group, whereas OST of animals was significantly increased to 33 and 51 days in the IL-13Rα2 DNA vaccine alone (P < 0.05) and ECDα2 boosted group (P < 0.01), respectively.