In HCT-116 cells, suppression of miR-31 decreased migration but increased invasion in vitro, this phenomenon might be partly explained by the fact that the migration assay detected the cell motility through an 8 μm pore polycarbonate membrane; however, the invasion assay not only evaluated cell motility but also the ability of tumor cells to invade through a basement membrane model (a reconstituted basement membrane matrix of proteins, including laminin and type IV collagen, derived from the Eangelbreth Holm-Swarm (EHS) mouse tumor [27]). This evidence concerns the gene LAMB2 and neoplasm.