However, as for the cell invasion, it had a stronger effect on HCT-116p53+/+ than HCT-116p53-/- cell line, the apoptotic rate in HCT-116p53+/+ was lower than that in HCT-116p53-/- cell line, suggesting miR-31 affected the colon cancer partly in a p53 dependent manner. The gene discussed is TP53; the disease is malignant colon neoplasm.