We used oligo-dT primed cDNA and a PCR strategy that would detect both DUX4-fl and DUX4-s (see Figure 1B) to determine the presence of polyadenylated DUX4 mRNAs in quadriceps muscle needle biopsies from ten FSHD and fifteen control individuals (Table 1 and Figure 1C). Here, DUX4 is linked to Facioscapulohumeral dystrophy.