Here we detected Bmi-1 at mRNA level by QRT-PCR method and found that Bmi-1 is overexpressed in gastric tumors and Bmi-1 overexpression correlates with tumor size, depth of invasion (T classification), or lymph node metastasis (N classification), which confirms previous observation of Bmi-1 at protein level. The gene discussed is BMI1; the disease is gastric neoplasm.