After confirming acute liver damage by the increased serum levels of alanine aminotransferase (ALT) (Fig. 1A), we analyzed the time-course of mRNA expression for synoviolin, Krüppel-like factor 6 (KLF6)—a key transcriptional factor contributing to liver fibrosis [29], α-SMA-activated HSC marker, and the collagen I gene (COL1A1) using real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) (Fig. 1B). This evidence concerns the gene ACTA1 and Hepatic fibrosis.