To determine if different signaling nodes in combination could provide superior stratification of FLT3-ITD and FLT3-WT AML samples, all possible pairs of the 80 signaling nodes with AUC of the ROC ≧0.7 or lowest p≤0.05 from study 1 (Table S3) were evaluated for their ability to improve stratification of the FLT3 receptor mutational status (Table S6). The gene discussed is FLT3; the disease is acute myeloid leukemia.