To understand how FLT3 receptor and NPM1 mutational status relate to intracellular biological pathways in diagnostic AML samples, we assessed IL-27 induced Jak/Stat signaling and FLT3L induced PI3K and Raf/Ras/MAPK signaling responses in FLT3 receptor and NPM1 molecular defined subgroups. This evidence concerns the gene IL27 and acute myeloid leukemia.