Cholesterol-loaded CCF-STTG1 astrocytoma cells were treated with approximately 50 μg/ml of apoE3-concentrated media or the equivalent of total protein from vector-concentrated media/ml (no detectable apoE) plus 5 μM T1317 or vehicle, and incubated in serum-free media for 48 hours. This evidence concerns the gene APOE and astrocytoma (excluding glioblastoma).