To evaluate DNA binding activity of overexpressed, nuclear STAT3/pSTAT3 and to confirm its constitutive activation in cervical cancer, we performed EMSA analysis of nuclear proteins derived from human cervical cancer cell lines, C33a, SiHa and CaSki and a subset of freshly collected biopsies from normal, precancer and cancer lesions. The gene discussed is STAT3; the disease is cervical cancer.