Blood plasma samples for this work were collected under the identical lab protocol from15 breast cancer patients (BCP) (ER positive, Her2 negative; invasive ductal carcinoma; 5 are stage I, 7 are stage II and 3 are stage III) and 15 control healthy persons (HP) of matched age, race, and menopausal status. The gene discussed is ESR1; the disease is invasive ductal breast carcinoma.