In mice, the requirement of CD8+ T cells for the control of L. major infection is shown to be partly dependent on the procedure of challenge: β2-microglobuine or CD8+ deficient C57BL/6 mice when challenged with high dose of L. major have the ability to cure the lesion, which indicates that CD8+ T cells are not necessary for the control of primary [44] infection, while in the intradermal challenge with low dose (100 metacyclic promastigotes into the ear dermis) the outcome of primary L. major infection in anti-CD8 Ab treated or CD8 deficient mice was dependent on the CD8+ T cells [45]. This evidence concerns the gene CD8A and infection.