MMP1 and melanoma: In order to validate the involvement of Mcol-A in EDP-induced melanoma invasion, B16F1 cells treated or not with EDPs were preincubated with a specific blocking anti-MMP-1 antibody (Brennan et al, 2003) (10 μg ml–1) and the invasiveness was assayed in vitro. As shown in Figure 4E, treatment of B16F1 and EDP-treated B16F1 with this antibody significantly decreased their invasive properties, reproducing the effect of galardin on B16F1 invasiveness after stimulation by EDPs.