F10 and hepatocellular carcinoma: To assess the importance of HSPG heparan sulfate (HS) sidechains in Ad5 cell binding and uptake mediated by interaction with human FX we pre-treated human HepG2 hepatoma and SKOV3 ovarian carcinoma cells with heparinase III prior to performing Ad5 cell attachment and gene transfer experiments in the presence or absence of FX.