Consistent with what has been observed in polarized intestinal monolayers [5], we found that a non-DAF binding CVB isolate (CVB-Nancy) was incapable of infecting HBMEC from the apical surface (Figure 3A) and DAF siRNA (Supplemental Figure S5) inhibited binding and infection by CVB (Supplemental Figure S2A). Here, CD55 is linked to infection.