To test the silencing efficiency of the miRNA-NG2, we transduced glioma cells, already expressing a PDGF-B/DS Red vector, with retroviral vectors expressing GFP and either the miRNA-NG2 or a negative control miRNA (miRneg, not complementary to any cellular mRNA) and, after 7 days, we compared the expression of NG2 in the two conditions. Here, PDGFB is linked to central nervous system cancer.