Cohen and coworkers demonstrated by analyses in C57BL/6 mice inoculated with B16 melanoma cells that DTA-1 treatment is more effective when given several days after tumor inoculation as compared to antibody treatment and tumor inoculation at the same day, suggesting that the efficacy of DTA-1 requires upregulation of GITR on tumor-activated T cells. This evidence concerns the gene TNFRSF18 and melanoma.