Carrying out the same low temperature treatment, the oocytes were stained with CREST (anticentromere antibody, autoimmune sera from patients with calcinosis, Raynaud phenomenon, esophageal dysmotility, sclerodactyly, and telangiectasia, a marker of CENPs at the kinetochores) and α-tubulin. The gene discussed is SS18L1; the disease is calcinosis.