TP53 and infection: To confirm that p53 knockdown and overexpression of oncogenic H-RasG12V induce malignant transformation of Barrett's epithelial cells, we infected a second telomerase-immortalized Barrett's epithelial cell line (BAR10-T) with pSuper-p53RNAi followed by infection of the population of p53RNAi-expressing cells (rather than a selected clone) with pBabe- H-RasG12V.