To confirm, by Western blot analysis, whether the novel CHD7 CRA_e transcript is in fact translated into a 100 kDa protein, we sub-cloned its coding sequence into the bicistronic lentiviral pLV-EGFP expression vector and overexpressed CHD7 CRA_e isoform in the DU145 prostate carcinoma cell line. The gene discussed is CHD7; the disease is prostate carcinoma.