Correspondingly, no significant amount of Ang-2 protein was detectable in cytosolic extracts and culture supernatants of various colon carcinoma cell lines (LS174T, HT29, DLD-1 and SW948) cultured under normoxia or hypoxia to mimic the conditions inside the tumour (Supplementary Figure 2). This evidence concerns the gene ANGPT2 and colon carcinoma.