Figures 4A and 5A show representative images of mycobacterial colocalization with LAMP-1 and CD63, respectively. Heat-killed MT103 and SO2 strain showed similar high levels of colocalization with LAMP-1 (70% and 80%, respectively), whereas virulent MT103 exhibited reduced colocalization (25%) (Figure 4B). However, in the case of CD63, a phagolysosome marker, both SO2 and MT103 revealed poor colocalization (35% and 20%, respectively) against the 75% colocalization observed in the infection with heat-inactivated MT103 (Figure 5B) over the period of 48 h. Here, CD63 is linked to infection.