To improve the therapeutic efficacy with IL-12 but simultaneously minimize the toxicity, several strategies have been created including targeting of IL-12 to only tumor, and coadministration with Treg cell-depleting antibodies such as anti-CD25 antibody, antibodies against immune suppressive signals such as cytotoxic T lymphocyte antigen (CTLA)-4 and IL-10, other cytokines, and anticancer drugs. This evidence concerns the gene IL10 and neoplasm.