To determine whether there was an association between promoter methylation status, protein and mRNA levels of RASSF1A and BLU, 69 NSCLC primary tumors (including 32 patients who had been analyzed by MSO) were assessed by immunohistochemistry (Fig. 3A), multiplex semi-quantitative RT-PCR (Fig. 3B) and methylation-specific PCR (Fig. 3C) assays. The gene discussed is ZMYND10; the disease is non-small cell lung carcinoma.