In addition, H1299 cancer cell treated with demethylating reagent (AZA), histone deacetylase inhibitor (TSA) and overexpression of CTCF led to restoration of CTCF binding as well as a change of chromatin structure separated by the presumable insulator sequence between RASSF1A and BLU loci (Fig. 6B). Here, CTCF is linked to cancer.