ANXA5 and neoplasm: Concomitantly, we have measured apoptosis and necrosis of the tumour cells; when incubated together with Th17 cells, we could detect a statistically significant increase of Annexin V and PI staining after 24 h in tumour cells incubated with Th17 cells (33.0% Annexin V positive and 10.8% PI positive) vs tumour cells alone (3.9% Annexin V positive and 6.7% PI positive), whereas the apoptosis and necrosis in case of the PBMC population without Th17 cells are significantly lower (10.8% Annexin V positive and 6.8% PI positive) with P<0.05 (Figure 5B).