An AD brain immunolabeled with no primary antibody and both an anti-mouse horseradish peroxidase (HRP) conjugated secondary and an anti-mouse alkaline phosphatase (AP) conjugated secondary reacted with both 3, 3′-Diaminobenzidine (DAB) and AP red illustrate the absence of non specific immunolabeling in the temporal cortex. The gene discussed is DHCR7-DT; the disease is Alzheimer disease.