For this, Western blotting to detect phosphorylated and total levels of the Rac1 effectors p21 activated kinase (PAK-1/2), the RhoA effector Rho kinase (ROCK), and downstream signaling molecules AKT and ERK was done on the pooled tumor extracts from 11 mice per genotype. The gene discussed is AKT1; the disease is neoplasm.