EPB41L1 and Familial prostate cancer: To investigate methylation as a potential cause of diminished EPB41L1 expression in cancer, 47 CpG sites around the transcriptional start site (from bp -208 to +157) were analyzed by bisulfite sequencing in the prostate cancer cell lines PC3 and LNCaP, in two prostate cancer tissues with lowered expression, one benign prostate sample and in leucocytes.