To investigate methylation as a potential cause of diminished EPB41L1 expression in cancer, 47 CpG sites around the transcriptional start site (from bp -208 to +157) were analyzed by bisulfite sequencing in the prostate cancer cell lines PC3 and LNCaP, in two prostate cancer tissues with lowered expression, one benign prostate sample and in leucocytes. This evidence concerns the gene EPB41L1 and prostate cancer.